A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture

ABSTRACT: Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases have gathered considerable excitement as a tool for genome engineering. However, questions remain about the specificity of their target site recognition. Most previous studies have examined predicted off-target binding sites that differ from the perfect target site by one to four mismatches, which represent only a subset of genomic regions. Here, we used ChIP-seq to examine genome-wide CRISPR binding specificity at gRNA-specific and gRNA-independent sites. For two guide RNAs targeting the murine Snurf gene promoter, we observed very high binding specificity at the intended target site while off-target binding was observed at 2- to 6-fold lower intensities. We also identified significant gRNA-independent

ORGANISM(S): Homo sapiens

SUBMITTER: Henriette O'Geen 

PROVIDER: E-GEOD-61099 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress