Role of Tet3 and DNA replication in zygotic demethylation of both paternal and maternal genomes [RRBS]

ABSTRACT: With the exception of imprinted genes and certain repeats, DNA methylation is globally erased during pre-implantation development. Recent studies have suggested that Tet3-mediated oxidation of 5-methylcytosine (5mC) and DNA replication-dependent dilution both contribute to global paternal DNA demethylation, but demethylation of the maternal genome occurs via replication. Here we present genome-scale DNA methylation maps for both the paternal and maternal genomes of Tet3-depleted and/or DNA replication-inhibited zygotes. In both genomes, we found that inhibition of DNA replication blocks DNA demethylation independently from Tet3 function, and that Tet3 facilitates DNA demethylation by coupling with DNA replication. For both, our data indicate that replication-dependent dilution is the major

ORGANISM(S): Mus musculus

SUBMITTER: Yi Zhang 

PROVIDER: E-GEOD-61330 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress