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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse embryonic stem (ES) cells to study the effect of Abca1 +/- mutations in ES cells.

ABSTRACT: effect of +/- mutations in ES cells

ORGANISM(S): Mus musculus

SUBMITTER: Derek Symula

PROVIDER: E-GEOD-614 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional annotation of mouse mutations in embryonic stem cells by use of expression profiling.

Symula Derek J DJ Zhu Yiwen Y Schimenti John C JC Rubin Edward M EM

Mammalian genome : official journal of the International Mammalian Genome Society 20040101 1

Expression profiling offers a potential high-throughput phenotype screen for mutant mouse embryonic stem (ES) cells. We have assessed the ability of expression arrays to distinguish among heterozygous mutant ES cell lines and to accurately reflect the normal function of the mutated genes. Two ES cell lines hemizygous for overlapping regions of mouse Chromosome (Chr) 5 differed substantially from the wildtype parental line and from each other. Expression differences included frequent downregulati ...[more]

PMID: 14727137

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