Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from mouse integration-free iPS cells

ABSTRACT: Reprogramming process can bring in genetic and epigenetic abnormalities, and these abnormalities can endow effect on gene expression of obtained iPSCs. We used Microarray to detect obvious gene expression differences between iPS cells ,which were induced using one episomal plasmid from C57BL/6 mouse embryonic fibroblasts, and one normal ES cell line from C57BL/6 inbred strain mice. iPS cells and control ES cells were selected in early passage for RNA extraction and hybridization on Affymetrix microarrays. To reduce the effect of in vitro culture, we chose iPS cells and ES cells in passage 7 to 10 for analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Libin Wang

PROVIDER: E-GEOD-61403 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

Expression data from mouse integration-free iPS cells

Project description:Reprogramming process can bring in genetic and epigenetic abnormalities, and these abnormalities can endow effect on gene expression of obtained iPSCs. We used Microarray to detect obvious gene expression differences between iPS cells ,which were induced using one episomal plasmid from C57BL/6 mouse embryonic fibroblasts, and one normal ES cell line from C57BL/6 inbred strain mice.

2015-09-14 | GSE61403 | GEO

Genomic alteration in MRC5-derived induced pluripotent stem (MRC5-iPS) cell line, Tic

Project description:Disease-specific induced pluripotent stem (iPS) cells have been used for a model to analyze pathogenesis of the disease. We generated iPS cells derived from a fibroblastic cell line of ataxia telangiectasia (AT-iPS cells). In analysis of AT-iPS cells, the human wild-type iPS cell line (MRC5-iPS) was generated and cultured in the same conditions as the diseased iPS cell lines. It is an ideal control cell line for the disease and patient-specific iPS cell lines. Because MRC5-iPS cells exhibited considerable chromosomal abnormalities in vitro, we performed a structural alteration analysis by using a SNP genotyping array for MRC5-iPS cell line, Tic, at passage 15, passage 30, and passage 37.

2013-07-23 | GSE49082 | GEO

Transcription profiling of human induced pluripotent stem cells free of exogenous DNA are derived with episomal vectors (super series)

Project description:Human induced pluripotent stem (iPS) cells have previously been derived from somatic cells using viral vectors that integrate transgenes into the genome. Genomic integration, however, can allow persistent leaky expression of the transgenes and can create insertional mutations, thus limiting the utility of these cells for both research and clinical applications. Here, we describe the derivation of human iPS cells free of vector and transgene sequences using non-integrating oriP/EBNA1-based episomal vectors. The resulting iPS cells are similar to human embryonic stem (ES) cells in both proliferative and developmental potential. These results demonstrate that reprogramming of human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors, and removes one important obstacle to the clinical applications of these cells. This SuperSeries is composed of the following subset Series:; GSE15175: Human induced pluripotent stem cells free of exogenous DNA are derived with episomal vectors (fig 1.c); GSE15176: Human induced pluripotent stem cells free of exogenous DNA are derived with episomal vectors (fig 4.a) Experiment Overall Design: Total 21 samples were analyzed to confirm the similarity of human iPS cells derived with episomal vectors with human ES cells, and a dissimilarity with fibroblasts. Experiment Overall Design: Refer to individual Series

2009-04-04 | E-GEOD-15148 | biostudies-arrayexpress

Gene expression data from teratomas formed by C57BL/6 (B6) ES and EiPS cells in B6 mice

Project description:Embryonic fibroblast from C57BL/6 (B6) mice were reprogrammed to EiPS without exogenous DNA integration using an single episomal vector. The EiPS cells and B6 ES cells were then transplanted into B6 mice to form teratomas. We used microarrays to profile the gene expression differences between the teratomas formed by B6 ES cells and EiPS cells.

2011-04-13 | GSE28573 | GEO

Genomic alteration in MRC5-derived induced pluripotent stem (MRC5-iPS) cell line, Tic

2013-07-23 | E-GEOD-49082 | biostudies-arrayexpress

Mus musculus

Project description:Genome sequencing of C57BL/6 iPS and ES cells

| PRJDB44 | ENA

Transcription profiling of human induced pluripotent stem cells free of exogenous DNA derived with episomal vectors

2009-04-04 | E-GEOD-15176 | biostudies-arrayexpress

Human induced pluripotent stem cells free of exogenous DNA are derived with episomal vectors (fig 4.a)

2009-03-27 | GSE15176 | GEO

Human induced pluripotent stem cells free of exogenous DNA are derived with episomal vectors (fig 1.c)

2009-03-27 | GSE15175 | GEO