Unknown,Transcriptomics,Genomics,Proteomics

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Cohesin regulates gene expression through spatial clustering of enhancer elements


ABSTRACT: H3K27Ac ChIP-seq in wild type and cohesin-deficient thymocytes Rad21 was deleted in CD4+ CD8+ double positive (DP) thymocytes by crossing a Rad21 floxed allele with a Cd4-driven Cre transgene. DP positive thymocytes were FACS-sorted from control and Rad21-/- littermates, which were then used to perform chromatin immunoprecipitation for histone H3 acetylated on lysine 27 (H3K27Ac).

ORGANISM(S): Mus musculus

SUBMITTER: Gopuraja Dharmalingam 

PROVIDER: E-GEOD-61428 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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