Isolation and functional characterization of the arteriole hematopoietic stem cell niche

ABSTRACT: Hematopoietic stem cells (HSCs) inhabit distinct microenvironments within the adult bone marrow (BM) that govern the delicate balance between HSC quiescence, self-renewal, and differentiation. It has been suggested that quiescent HSCs localize adjacent to BM arteriole endothelial cells in a significant and non-random distribution. This data suggests that the arteriole BM vascular niche may be the primary HSC niche. Because the BM arteriole niche is composed of tightly-associated pericytes, including smooth muscle actin+, LepR+, Nestin+, NG2+, and nonmyelinating Schwann cells, we sought to begin to uncouple the arteriole BM EC niche by examining its capacity to support the maintenance and expansion of HSCs ex vivo and in vivo. We developed a method to isolate and culture BM arteriole endoth

ORGANISM(S): Mus musculus

SUBMITTER: Olivier Elemento 

PROVIDER: E-GEOD-61636 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress