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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Deep sequencing of microRNA present in ejaculated bull spermatozoa

ABSTRACT: The objectives of our study were to identify microRNA (miRNA) present in bovine sperm. Angus bulls (n=6) were assigned to treatments of either toxic or non-toxic fescue seed diets. Semen was collected and subjected to RNA isolation, enriched for miRNA. Three samples from each treatment group were chosen based on RNA quality and pooled for deep sequencing.

ORGANISM(S): Bos taurus

SUBMITTER: Scott Pratt

PROVIDER: E-GEOD-61746 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The bull sperm microRNAome and the effect of fescue toxicosis on sperm microRNA expression.

Stowe Heather M HM Calcatera Samantha M SM Dimmick Marcy A MA Andrae John G JG Duckett Susan K SK Pratt Scott L SL

PloS one 20141202 12

Tall fescue [Schedonorus phoenix (Scop.) Holub] accounts for nearly 16 million hectares of pasture in the Southeastern and Mid-Atlantic U.S. due to its heat, drought, and pest resistance, conferred to the plant by its symbiotic relationship with the endophyte Neotyphodium coenophialum. The endophyte produces ergot alkaloids that have negative effects on the growth and reproduction of animals, resulting in the syndrome known as fescue toxicosis. The objectives of our study were to identify microR ...[more]

PMID: 25462855

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