Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum

ABSTRACT: Development of the soil amoeba Dictyostelium discoideum is triggered by starvation. When placed on a solid substrate, the starving solitary amoebae cease growth, communicate via extracellular cAMP, aggregate by tens of thousands and develop into fully integrated multicellular organisms. Early phases of the developmental program are often studied in cells starved in suspension while cAMP is provided exogenously. Previous studies revealed massive shifts in the transcriptome under these developmental conditions and a close relationship between gene expression and morphogenesis, but were limited by the sampling frequency and the resolution of the methods. Here, we combine the superior depth and specificity of RNA-seq with high frequency sampling during filter development and cAMP pulsing in su

ORGANISM(S): Dictyostelium discoideum

SUBMITTER: Gad Shaulsky 

PROVIDER: E-GEOD-61914 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress