Global identification of target recognition and cleavage by the Microprocessor in human ESCs.

ABSTRACT: The Microprocessor plays an essential role in canonical miRNA biogenesis by facilitating cleavage of stem-loop structures in primary transcripts to yield pre-miRNAs. Although miRNA biogenesis has been extensively studied through biochemical and molecular genetic approaches, it has yet to be addressed to what extent the current miRNA biogenesis models hold true in intact cells. To address the issues of in vivo recognition and cleavage by the Microprocessor, we investigate RNAs that are associated with DGCR8 and Drosha by using immunoprecipitation coupled with next-generation sequencing. Here, we present global protein-RNA interactions with unprecedented sensitivity and specificity. Our data indicate that precursors of canonical miRNAs and miRNA-like hairpins are the major substrates of the

ORGANISM(S): Homo sapiens

SUBMITTER: Young-Soo Kwon 

PROVIDER: E-GEOD-61979 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress