Maintenance and resurrection of retinal pigmented epithelial cell phenotype by type 1 TGF-beta receptor kinase inhibitors

ABSTRACT: Age-related macular degeneration (AMD) is a leading cause of blindness. Most vision loss occurs following the transition from a disease of deposit formation and inflammation to a disease of neovascular fibrosis and/or cell death. Here, we investigate how protracted wound stimulus leads to seminal changes in gene expression and the onset of a self-sustained state of wound response in retinal pigmented epithelial (RPE) cells. Using a human fetal RPE cell culture model and a systems level transcriptome analysis, we show that prolonged subconfluent culture resulting from repeated passage, leads to terminal acquisition of a mesenchymal-like phenotype post-confluence accompanied by altered expression of >40% of the transcriptome. In contrast, at subconfluence <5% of transcripts have >2-fold exp

ORGANISM(S): Homo sapiens

SUBMITTER: Monte Radeke 

PROVIDER: E-GEOD-62224 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress