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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome Analysis of KSHV virions produced from BCBL1 and BAC36 in 293L cells

ABSTRACT: The objective of this study was to identify the viral transcripts packaged into the virion particles produced from BCBL1 cells as well as virions from 293L cells containing BAC36 BACs RNA from virions were extracted and analyzed by RNA sequencing

ORGANISM(S): Human herpesvirus 8

SUBMITTER: Subhash Verma

PROVIDER: E-GEOD-62343 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome Analysis of KSHV virions produced from BCBL1 and BAC36 in 293L cells

Project description:The objective of this study was to identify the viral transcripts packaged into the virion particles produced from BCBL1 cells as well as virions from 293L cells containing BAC36 BACs

2014-10-16 | GSE62343 | GEO

Transcriptome Analysis of KSHV during de novo primary infection of human B-and endothelial-cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Profiling selective packaging of host RNA and viral RNA modification in the SARS-CoV-2 virion

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2022-03-06 | GSE182883 | GEO

Transcriptome Analysis of KSHV during de novo primary infection of endothelial (TIVE) cells

Project description:The objective of this study was to identify the viral transcripts packaged into the virion particles and the transcription profiles of the viral genome during early infection, till the virus establishes latent infection in endothelial (TIVE) cells Samples from each time points were analyzed and the experiments were done in duplicate.

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Transcriptome Analysis of KSHV during de novo primary infection of human B cells (PBMCs)

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An Updated overview of the Virion Proteome of Kaposi Sarcoma Associated Herpesvirus

Project description:The virion proteins of Kaposi Sarcoma Associated Herpesvirus (KSHV) were initially characterized in 2005 in two separate studies that combined detected 24 viral proteins and a few cellular components via LC-MS/MS or MALDI-TOF. Despite considerable advances in the sensitivity and specificity of mass spectrometry instrumentation in recent years, leading to significantly higher yields in detections, the KSHV virion proteome has not been revisited. In this study, we have re-examined the protein composition of purified KSHV virions via Ultra-High Resolution Qq-Time-Of-Flight mass spectrometry (UHR-QqTOF). Our results confirm the detection of all previously reported virion proteins, in addition to 17 other viral proteins, some of which have been characterized as virion-associated using other methods, and 10 novel proteins identified as virion-associated for the first time in this study. These results add KSHV ORF9, ORF23, ORF35, ORF48, ORF58, ORF72/vCyclin, K3, K9/vIRF1, K10/vIRF4, and K10.5/vIRF3 to the list of KSHV proteins that can be incorporated into virions. The addition of these proteins to the KSHV virion proteome provides novel and important insight into early events in KSHV infection mediated by virion-associated proteins.

2020-12-10 | PXD022626 | Pride

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