Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

12 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile of GM-CSF derived bone marrow dendritic cell subsets

ABSTRACT: GM-CSF derived bone marrow cultures contain several subsets of CD11c+MHCII+ mononuclear phagocytes Using Affymetrix microarrays we compared gene expression of the different mononuclear phagocytes within the bone marrow culture The Affymetrix GeneChip Mouse Gene 1.0 arrays were used to define gene expression profiles in the different population.

ORGANISM(S): Mus musculus

SUBMITTER: Julie Helft

PROVIDER: E-GEOD-62361 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profile of GM-CSF derived bone marrow dendritic cell subsets after LPS stimulation

Project description:GM-CSF derived bone marrow cultures contain several subsets of CD11c+MHCII+ mononuclear phagocytes Using Affymetrix microarrays we compared gene expression of the different mononuclear phagocytes within the bone marrow culture. The different populations are left unstimulated or are stimulated with LPS The Affymetrix GeneChip Mouse Gene 1.0 arrays were used to define gene expression profiles in the different population.

2015-09-01 | E-GEOD-62746 | biostudies-arrayexpress

Gene expression profile of GM-CSF derived bone marrow dendritic cell subsets

2015-02-27 | GSE62361 | GEO

Gene expression profile of GM-CSF derived bone marrow dendritic cell subsets after LPS stimulation

2015-09-01 | GSE62746 | GEO

Global gene expression profiling using total RNA [BMM, MPI and SPG]

Project description:Differentiation of myeloid progenitor cells leads to distinct populations of mononuclear phagocytes.Various macrophages exhibit distinct biological properties. Here wanted to delineate and characterize gene expression patterns in bone marrow derived macrophages Here wanted to delineate and characterize gene expression patterns in two newly established, self-renewing, in vitro grown non-transformed lines (MPI cells) and in the SP37A3 immature myeloid dendritic cell line. We used microarrays to detail the global programme of gene expression in MPI cells and to compare them to other types of mononuclear phagocytes. [BMM] Total cellular RNA from ex vitro differentiated bone marrow derived macrophages was extracted and hybridized to Affymetrix microarrays to carry out cluster analysis and overlap and gene set analysis for enriched pathways. [MPI and SPG] Total cellular RNA from independently established MPI lines and a splenic immature dendritic cell line was extracted and hybridized to Affymetrix microarrays to carry out cluster analysis and overlap and gene set analysis for enriched pathways.

2013-06-08 | E-GEOD-47473 | biostudies-arrayexpress

The dural innate immune barrier protects the CNS from systemic virus invasion [MHCII_10X]

Project description:To investigate heterogeneity of MHCII high CNS border-associated mononuclear phagocytes (BAMPs), MHCII high BAMPs from 30 adult male mice were FACS sorted and single-cell RNA seqs were performed

2022-07-25 | GSE209574 | GEO

RNA-seq of of bone marrow derived mononuclear phagocytes

Project description:RNA-seq of of bone marrow derived mononuclear phagocytes

| PRJEB26391 | ENA

Microarray analysis of WT bone marrow myeloid progenitors, BM cultured with GM-CSF and M-CSF, and monocytes treated with GM-CSF

Project description:Analysis of genes induced in DC precursors and in BM cells and monocytes treated with GM-CSF For progenitor arrays, bone marrow progenitors (CMP, GMP, CDP, and pre-cDC) were harvested from WT C57Bl/6 mice. For culture arrays, BM was cultured in the presence of GM-CSF or M-CSF and adherent and non-adherent cells sorted. For monocyte cultures, sorted BM monocytes were treated with GM-CSF for 0, 24 or 48 hours.

2012-05-20 | E-GEOD-37029 | biostudies-arrayexpress

Gene expression microarray data of bone marrow derived macrophages using GM-CSF(GM-BMMs) co-cultured with E0771 breast tumor cells

Project description:E-FABP is dominately expressed in macrophages/dendritic cells. Thus E-FABP may play a siginificant role in their immune response to tumor insult. We treated mouse GM-BMMs of different genotype with E0771 breast cancer cells. Then we investigate the global gene expression in these GM-BMMs in response to tumor treatment. Bone marrow cells were collected from naïve C57BL/6 and E-FABP knockout mice of 6~8 week old. GM-BMMs were derived using GM-CSF (in RPMI1640 with 5%FBS) for 7 days before tumor treatment. After 18hr tumor teatment, GM-BMM total RNA was extracted using RNeasy Mini Kit (Qiagen) and subjected to mouse mRNA Gene expression by Affymetrix microarray.

2014-09-02 | E-GEOD-54073 | biostudies-arrayexpress

Microarray analysis of therapeutic (GM4) and non therapeutic (GM) NOD DC

Project description:We have previously demonstrated that bone marrow-derived DC can prevent diabetes development and halt progression of insulitis in NOD mice, the mouse model of type 1 diabetes (T1D). The DC population that was most effective in this therapy had a mature phenotype, expressed high levels of costimulatory molecules and secreted low levels of IL-12p70. The protective DC therapy induced regulatory Th2 cells that shifted the dominant Th1 environment, present in NOD mice, to a mixed Th1/Th2 milieu. Microarray analysis of therapeutic and non-therapeutic DC populations revealed several novel molecules that could play important roles in the observed DC-mediated therapy. The therapeutic DC population expressed a unique pattern of costimulatory molecules and chemokines, which were confirmed by flow cytometry and ELISA assays. We have performed in vitro chemotaxis assays that demonstrated the therapeutic DC preferentially attracted Th2 cells, as compared to Th1, Treg or naÃ¯ve T cells. In addition we quantified the in vivo migration of activated islet-specific T cells to the pancreas using novel cell labeling techniques and 19F nuclear magnetic resonance. A subcutaenous injection of therapeutic DC alters the migration of both Th1 and Th2 cells to the pancreas, and Th1 cells appeared in the lymph node draining the site of DC injection. These results suggest that the therapeutic function of DC is mediated in part by the chemoattractive properties of these DC for diabetogenic Th1 cells. Bone marrow cells from NOD mice were cultured in GM or GM + IL-4 to generate DC. DC were purified on metrizamide gradients after 4 days of culture. Purified DC were stimulated with LPS + IFNg and RNA taken 0, 6 and 20 hours after stimulation. Three biological replicates were performed but not all RNA samples were good. The are 3 replicates for GM/0, GM4/0, GM4/6 and 2 replicates for GM/6, GM/20 and GM4/20

2010-07-29 | E-GEOD-15320 | biostudies-arrayexpress

Gene expression data from wild-type and Nlrc5 knockout GM-CSF induced bone marrow dendritic cells infected with Newcastle Disease virus.

Project description:Nlrc5 is encoding a Nod-like receptor protein NLRC5/NOD27. To check the involvement of Nlrc5 in antiviral response, we examined gene expression profile in wild-type and Nlrc5 knockout GM-CSF bone marrow macrophage with using microarrays. Bone marrow cells were cultured with GM-CSF and harvested on day 6. The induced dendritic cells were then stimulated with NDV at moi 1, collected at 0, 2, and 4 hours after infection. Total RNA was extracted and subjected to microarray experiment according to manufactures' instructions.

2010-12-22 | E-GEOD-22448 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data