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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Differential gene expression in erythroid progenitor cells from β-thalassaemia patients and healthy controls [Bioconductor/limma R analysis]

ABSTRACT: This study uses microarray technology to examine the erythroid progenitor mRNA of patients with transfusion dependent β-thalassaemia and compare it to erythroid progenitor mRNA from healthy controls. We observed no statistical difference in gene expression between the groups following 7 days in culture. However, following 14 days in culture we observed differential expression of 161 genes. Haematopoietic cells from the peripheral blood of 6 β-thalassaemia patients and 6 healthy controls was grown in semi-solid media. After 7 and 14 days in culture cells of erythroid origin were isolated. Total RNA was isolated from these for microarray gene expression analysis

ORGANISM(S): Homo sapiens

SUBMITTER: Luke Forster

PROVIDER: E-GEOD-62430 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential gene expression in erythroid progenitor cells from β-thalassaemia patients and healthy controls [Bioconductor/limma R analysis]

Project description:This study uses microarray technology to examine the erythroid progenitor mRNA of patients with transfusion dependent β-thalassaemia and compare it to erythroid progenitor mRNA from healthy controls. We observed no statistical difference in gene expression between the groups following 7 days in culture. However, following 14 days in culture we observed differential expression of 161 genes.

2014-10-28 | GSE62430 | GEO

Differential Gene Expression Analysis in Early and Late Erythroid Progenitor Cells in β-thalassaemia

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-10-28 | E-GEOD-62431 | biostudies-arrayexpress

Differential gene expression in erythroid progenitor cells from β-thalassaemia patients and healthy controls [BRB-ArrayTools analysis]

2014-10-28 | GSE56088 | GEO

Comprehensive analysis of human proteomic erythroid progenitors

Project description:CD34-positive cells from peripheral blood were culture for 5 days in erythroid differentiating medium. Four progenitor stages were sorted by FACS using the established cell surface markers CD34 and CD36 with CD117, CD71, and CD105 (Yan H, Am J Hematol, 2021). A comprehensive analysis of the proteome of these four erythroid progenitor stages was done in quadruplicate using a label free proteomic approach.

2023-10-30 | PXD042615 | Pride

Differential gene expression in erythroid progenitor cells from ?-thalassaemia patients and healthy controls [BRB-ArrayTools analysis]

Project description:This study uses microarray technology to examine the erythroid progenitor mRNA of patients with transfusion dependent ?-thalassaemia and compare it to erythroid progenitor mRNA from healthy controls. We observed no statistical difference in gene expression between the groups following 7 days in culture. However, following 14 days in culture we observed differential expression of 277 genes. Haematopoietic cells from the peripheral blood of 6 ?-thalassaemia patients and 6 healthy controls was grown in semi-solid media. After 7 and 14 days in culture cells of erythroid origin were isolated. Total RNA was isolated from these for microarray gene expression analysis

2014-10-28 | E-GEOD-56088 | biostudies-arrayexpress

Exosc8-Regulated Genes in Fetal Liver-Derived Erythroid Progenitor Cells

Project description:Exosc8 and Exosc9 are components of the exosome that establish a barricade to erythroid maturation. Here, we knocked down Exosc8 in fetal liver-derived erythroid progenitor cells to determine the cohort of Exosc8-regulated genes in erythroid cells. Freshly isolated fetal liver progenitor cells were infected with retrovirus expressing shRNA targeting either luciferase or Exosc8. Total RNA was isolated from these cells after 3 days ex-vivo culture, during which the cells underwent erythroid maturation.

2014-08-09 | E-GEOD-60136 | biostudies-arrayexpress

Tiled expression analysis of human two phase primary erythroid culture

Project description:The transcription, mainly across the beta globin locus, was assessed of human two phase primary erythroid culture using a Nimblegen microarray over a set number of days following the initiation of differentiation

2018-06-14 | E-MTAB-6956 | biostudies-arrayexpress

renal damage in 24w2k1c v cont24w

Project description:The experiment was designed to study the effects of blood pressure on gene expressions in the kidney cortex. Non-clipped kidney cortex was harvested from eight 2k1c rats and eight sham rats after 4 and 24 weeks of clipping. The 2k1c is the experimental group which underwent a two kidney, one clip (2k1c) operation (renal artery stenosis), wheras the sham group serves as a control where no stenosis were performed.

2014-11-11 | E-MTAB-1449 | biostudies-arrayexpress

Ageing-rats-2011

Project description:The experiment was designed to study the effects of senescence on gene expressions in the kidney cortex. Kidney cortex was harvested from five rats at ten weeks of age and five rats at ninety weeks of age. Both kidneys were used and combined as biological replicates in the final analysis.

2012-03-28 | E-MTAB-613 | biostudies-arrayexpress

Leukemia associated mutant Wilms' tumour gene 1 protein promotes expansion of human hematopoietic progenitor cells

Project description:Analysis of gene expression levels in hematopoietic progenitor cells retrovirally transduced with full-length WT1(+/-) or a WT1-mutant lacking zinc-finger, WT1(delZ) cultured in vitro for 14 days. The hypothesis tested in this study was that the WT1-mutant confers an increased proliferation rate after 14 days in culture and an erythroid phenotype. Results provide information of upregulation of some genes associated with cellcycle progression, upregulated genes connected with erythropoiesis and downmodulation of some genes associated with myeloid differentiation. Total RNA obtained from CD34+ progenitor cells in suspension cultures for 14 days.

2013-06-21 | E-GEOD-47560 | biostudies-arrayexpress

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