Integrated molecular portrait of triple negative breast tumors from Mexican women using archived clinical samples

ABSTRACT: Triple negative breast tumours from archived formalin fixed paraffin embeded samples of the National Cancer Institute of Mexico were analyzed for differential gene expressión. Transcriptomic analysis of the 12 tumor samples was done with the FFPE-designed WG-DASL HT assay (Illumina) according to manufacturer’s instructions. This assay measures 29,285 annotated transcripts derived from the RefSeq database corresponding to 20,727 unique genes. Briefly, 200ng of total RNA were reverse-transcribed into biotinylated cDNA, which was then primer-extended with the Assay Specific Oligos. The cDNA was then amplified with universal primers and hybridized to Illumina Human WG DASL HT Expression BeadChip arrays. The Illumina Genome Studio V2010.2 was used to obtain the signal values (AVG-Signal), with

ORGANISM(S): Homo sapiens

SUBMITTER: Magali Olivier 

PROVIDER: E-GEOD-62502 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress