The RSC Complex localizes to coding sequences to regulate Pol II and histone occupancy (Agilent)

ABSTRACT: ATP-dependent chromatin remodelers regulate chromatin structure during multiple stages of transcription. We report that RSC, an essential chromatin remodeler, is recruited to the open reading frames (ORFs) of actively transcribed genes genome-wide, suggesting a role for RSC in regulating transcription elongation. Consistent with such a role, Pol II occupancy in the ORFs of weakly transcribed genes is drastically reduced upon depletion of the RSC catalytic subunit Sth1. RSC inactivation also reduced histone H3 occupancy across transcribed regions. Remarkably, the strongest effects on Pol II and H3 occupancy were confined to the genes displaying the greatest RSC ORF enrichment. Additionally, RSC recruitment to the ORF requires the activities of the SAGA and NuA4 HAT complexes and is aided by

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Chhabi Govind 

PROVIDER: E-GEOD-62519 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress