Expression data roots of Arabidopsis plants inoculated with Verticillium longisporum

ABSTRACT: Identification of genes differentially expressed in roots of Arabidopsis Col-0 and ndr1-1 mutants 48 h post inoculation with the fungal pathogen Verticillium longisporum. Arabidopsis Col-0 and ndr1-1 seeds were grown in petri dishes with liquid MS medium supplemented with 0.2% sucrose. After two weeks on a horizontal shaker (40 rpm), plants were rinsed twice in sterile distilled water and transferred to liquid 0.5xMS medium without sucrose, supplemented with 200 conidia ml-1 of V. longisporum isolate VL1 (CBS110220). Control plants were grown in 0.5x MS only. At 48 hours post inoculation, materials were harvested in biological replicates (>50 plant roots) and immediately frozen in liquid nitrogen. RNA was extracted with Aurum Total RNA Mini Kit (Bio-Rad) and hybridized to the Arabidopsis A

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jonas Roos 

PROVIDER: E-GEOD-62537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress