Characterization of the inflammatory phenotype of Mycobacterium avium subspecies paratuberculosis

ABSTRACT: Understanding the pathogenic mechanisms and host responses to Johne's disease, caused by Mycboacterium avium subspecies paratuberculosis (MAP), is complicated by a number of challenges of the disease progression and lack of ex vivo models to study the disease. We describe novel cell culture passage model which mimics the course of infection in vivo. Using this model we determined that cell culture passaged MAP develops an inflammatory phenotype initiating higher levels of pro-inflammatory cytokines and chemokines compared to levels initiated by non-passaged MAP. To verify that the MAP phenotypes were changing, we conducted transcriptome analysis of each population during the passage model and identified a change in gene expression, specifically and upregulation of genes involved in the

ORGANISM(S): Mycobacterium avium subsp. paratuberculosis

SUBMITTER: Luiz Bermudez 

PROVIDER: E-GEOD-62566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress