Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

15 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from prostate cancer cell lines

ABSTRACT: We have investigated Perineural invasion (PNI) and epithelial-mesenchymal transition (EMT) related gene expression profiles of PC cell lines. In this dataset, we include the expression data obtained from laminin binding O-glycan high or low androgen-independent PC cells. These data are used to obtain 70 genes that are differentially expressed in response to cell migraton signal via laminin receptors. 4 Total samples were analyzed. We generated the following pairwise comparisons using GeneSpring Gx Version12.6: PC3-H vs PC3-L; LNCaP vs LNCaP-AI. Genes with an FDRâ¤10% and a fold-change â¥2 were selected.

ORGANISM(S): Homo sapiens

SUBMITTER: Tohru Yoneyama

PROVIDER: E-GEOD-62576 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Lapatinib antitumor effect is associated with PI3K and MAPK pathway: an analysis in human and canine prostate cancer cells

Project description:The aberrant activation of HER2 has a pivotal role in bone metastasis implantation and progression in several tumor types, in-cluding prostate cancer (PC). Trastuzumab and other anti-HER2 therapies, such as lapatinib, have been used in human breast cancer HER2 positive. Although HER2 overexpression has been reported in PC, anti-HER2 therapy response has revealed conflicting results. We investigated the potential of lapatinib in inhibiting cell migration and inducing apoptosis in two human (LNCaP and PC3) and two canine PC cell lines (PC1 and PC2). Cell migration and apoptosis were evaluated by Annexin V/PI analysis after lapatinib treatment. The transcriptome analysis of all cell lines before and after treatment with lapatinib was also performed. We found increased apoptosis and migration inhibition in LNCaP cells (androgen-sensitive cell line), while PC1, PC2, and PC3 cells showed no alterations after the treatment. The transcriptome analysis of LNCaP and PC3 cell lines showed 158 dysregulated transcripts in common, while PC1 and PC2 cell lines presented 82. At the doses of lapatinib used, we observed transcriptional modifications in all cell lines. PI3K/AKT/mTOR pathway were enriched in human PC cells, while canine PC cells showed en-richment of tyrosine kinase antitumor response and HER2-related pathways. In canine PC cells, the apoptosis failed after lapatinib treatment, possibly due to the downregulation of MAPK genes. Prostate cancer cells insensitive to androgens may be resistant to lapatinib through PI3K gene dysregulation. The association of lapatinib with PI3K inhibitors may provide a more effective anti-tumor response and clinical benefits to PC patients.

2023-11-30 | E-MTAB-13551 | biostudies-arrayexpress

Lapatinib antitumor effect is associated with PI3K and MAPK pathway: an analysis in human and canine prostate cancer cells

2023-11-30 | E-MTAB-13552 | biostudies-arrayexpress

Toxicogenomics on mice liver of coumarins from Calophyllum brasiliense

Project description:A toxicogenomic analysis from liver of different pharmacological active coumarins (mammea A/BA+A/BB 3:1 and soulatrolide ) was performed on mice treated (20mg/kg/daily) for a whole week to evaluate if such compounds possess or could develop a hazardous profile on liver. In this dataset, we include the expression data obtained from dissected liver mouse treated with active coumarins (obtained from Calophyllum brasiliense) and control (SSI) for a whole week. Livers from 4 animals per treatment and control were subjected to RNA extraction. These data showed 46 genes up and 72 downregulated genes for mammea coumarins and 665 up and 1077 downregulated genes . Expression data from liver of mice treated with different coumarins (mammea and soulatrolide) from Calophyllum brasiliense 12 Total samples were analyzed. Liver from 4 animals per treatment groups were subjected to mRNA expression analysis. We generated the statistical computing and metrics in the arrayQualityMetrics data were normalized with RMA method and differential expressed genes obtained with Ingenuity Software. Genes with an FDRâ¤10% and a fold-change â¥2 were selected.

2015-09-06 | E-GEOD-72755 | biostudies-arrayexpress

Long noncoding RNA as putative biomarkers for protstate cancer detection [Set 2]

Project description:To identify which lncRNAs are differentially expressed in prostate cancer, the total RNA from prostate cancer cell lines (PC3, LNCaP), normal epithelial prostatic cells and the pool of 10 prostate tumor tissues and 10 adjacent normal prostate tissues were screened using SurePrint G3 human lncRNA microarrays (Agilent). The SurePrint G3 Human Gene array contains 16,472 lincRNAs and 34,127 mRNA genes. Gene expression in prostate cell lines and pooled tissue samples was measured. Three independent experiments (biological replicates) for cell lines (epithelial cells, PC3 and LNCap) and two independent experiments (technical replicates) for pooled prostate tissues (tumor and adjacent normal) samples were performed.

2014-10-17 | E-GEOD-55909 | biostudies-arrayexpress

Effects of prostate cancer patient urinary extracellular vesicles and hypoxic extracellular vesicles from prostate cancer cell lines on the transcriptomes of cancer-associated and normal fibroblasts

Project description:Patient-derived prostate fibroblast primary cultures PCF-54 and PCF-55 were established from two specimens of PC tissues. Urinary EVs were isolated from urine samples of 3 patients with PC and 2 healthy males and used for the treatment of prostate fibroblast primary cultures and normal foreskin fibroblasts. Normoxic and hypoxic EVs were isolated from cell culture medium of PC3 and LNCaP prostate cancer cell lines, cultivated in normoxic and hypoxic conditions respectively. The EV-treated fibroblasts were subjected to RNA sequencing analysis.

2023-04-01 | E-MTAB-11317 | biostudies-arrayexpress

Research of neopeptides/neoproteins in prostate cancer cell lines

Project description:Validation, by mass spectrometry (MS), of predicted neoantigens in urinary extracellular vesicles coming from cancer prostate patients. We performed MS on PC3, LNCaP and HCT116 cells and compared MS-detected peptides with established PC3 lncRNA peptides, peptides from lncRNAs upregulated in uEVs and Swiss prot databanks.

2022-05-31 | PXD032264 | Pride

MiR-23b/-27b Targets in Prostate Cancer Cell Lines

Project description:Analysis of genes regulated by miR-23b/-27b overexpression in aggressive PC3-ML cells, confirmed by antagomiR inhibition of miR-23b and miR-27b in the relatively indolent cell line LNCaP. Genes that were downregulated in PC3-ML overexpression and upregulated with LNCaP inhibition were further explored as downstream targets of miR-23b/-27b.

2016-02-10 | GSE76620 | GEO

Expression data from prostate cancer cell lines

Project description:We have investigated Perineural invasion (PNI) and epithelial-mesenchymal transition (EMT) related gene expression profiles of PC cell lines. In this dataset, we include the expression data obtained from laminin binding O-glycan high or low androgen-independent PC cells. These data are used to obtain 70 genes that are differentially expressed in response to cell migraton signal via laminin receptors.

2017-04-30 | GSE62576 | GEO

BV02 perturbed proteome (prostate cancer models)

Project description:short term response of prostate cancer models LNCaP clone FGC and PC-3 to BV02 treatment.

2018-01-16 | MSV000081938 | MassIVE

RNA-Seq analysis of prostate cancer cells after 3-bromopyruvic acid treatment

Project description:We performed RNA-seq analysis using differnt prostate cancer cell lines (PC3 and LNCaP) after 3-bromopyruvic acid treatment. We reported that 3-bromopyruvic acid regulatescell cycle-associated genes in PC3 cells. 3-bromopyruvic acid induces apoptosis and suppresses cell cycle progration, cell migration and invasion in PC3 cells.

2021-07-21 | GSE180328 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data