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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression study between dental pulp cells from TRPC6-mut individual and control individuals

ABSTRACT: As TRPC6 channel induces CREB-mediated trancription, Dental pulp cells from TRPC6-mut patient and from 6 controls were analyzed in order to verify if the disruption of TRPC6 leads to transcriptional changes. Cells were grown until reach 80-90% of confluency in DMEM/F12 media supplemented with 15% fetal bovine serum (Hyclone, TX), 1% penicillin/streptomycin, and 1% non-essential amino acids and maintained under standard conditions (37°C, 5% CO2). RNAs samples were extracted from cells in passage 5 to 7.

ORGANISM(S): Homo sapiens

SUBMITTER: Alysson Muotri

PROVIDER: E-GEOD-62620 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression study between dental pulp cells from TRPC6-mut individual and control individuals

Project description:As TRPC6 channel induces CREB-mediated trancription, Dental pulp cells from TRPC6-mut patient and from 6 controls were analyzed in order to verify if the disruption of TRPC6 leads to transcriptional changes.

2014-10-23 | GSE62620 | GEO

Expression study of human embryonic stem cells, dental pulp cells (DPCs) and induced pluripotent stem cells (iPSC) obtained from DPC for characterization of iPSC

Project description:iPSC were obtained from DPC from TRPC6-mut patient, a idiopathic autistic patient and a control. Original DPC and iPSC obtained were submited to expression analysis in order to check if the expression pattern obtained for the iPSC cells were closer related to embyonic cells than to the original DPC DPCs were grown until reach 80-90% of confluency in DMEM/F12 media supplemented with 15% fetal bovine serum (Hyclone, TX), 1% penicillin/streptomycin, and 1% non-essential amino acids and maintained under standard conditions (37°C, 5% CO2). RNA samples were extracted from cells in passage 5 to 7. iPSC were grown until reaches 70% of confluency in mTSER medium (Stem Cells Technologies). RNA samples were extracted from cells in passage 12 to 15.

2014-10-23 | E-GEOD-62622 | biostudies-arrayexpress

Modeling non-syndromic autism and the impact of TRPC6 disruption in human neurons

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-10-23 | E-GEOD-62632 | biostudies-arrayexpress

SAHA Rat Dental Pulp

Project description:Transcriptional response of rat dental pulp cells (DPCs) cultured with SAHA at early and late mineralisation time points Transcript profiling of DPC identified several novel genes expression induced and supressed by HDACi at 24 hrs and 14 days under mineralising conditions. SAHA induces several members of the MMP family of endopepsidases (TIMP-1, MMP-9, MMP-13) and other members of the endochondral ossification pathway at 24 h. 8 experiemental parameters were analysed, each carried out in quadruplicate

2015-06-16 | E-GEOD-67175 | biostudies-arrayexpress

SAHA Rat Dental Pulp

2015-06-16 | GSE67175 | GEO

Gene expression analysis of human permanent dental pulp

Project description:Gene expression analysis data of human permanent dental pulp tissues

2016-11-22 | GSE75644 | GEO

Comparative gene expression analysis of the human dental pulp in deciduous and permanent teeth

Project description:Human deciduous and permanent teeth exhibit different developmental processes, morphologies, histological characteristics and life cycles. In addition their pulp tissues react differently to external stimuli, such as the pulp sensitivity test, dental trauma and pulp therapy materials. These differences are attributable to their genetic backgrounds. Therefore the purpose of this study is to compare the differences of dental pulp in deciduous and permanent teeth. Pulp samples were obtained from permanent premolars (n=6, aged 11-14 years) and deciduous teeth (n=6, aged 11-14 years). Comparative cDNA microarrary analysis revealed several differences in gene expression between the deciduous and permanent pulp tissues. Each GSM record represents a pulp sample pooled from two teeth samples.

2014-08-21 | E-GEOD-51449 | biostudies-arrayexpress

Effect of EtOH on transcriptome signatures in human dental pulp stem cells

Project description:We have performed gene expression microarray analysis to profile transcriptomic signatures affected by EtOH in human dental pulp stem cells Established human dental pulp stem cells were treated with different dose of EtOH (0, 1, 5, 10, 20 and 50mM) for a different time periods (24 and 48 hrs). Total RNA was extracted and subjected to gene expression microarray analysis using Affymetrix human genome 2.0 plus array

2014-05-03 | E-GEOD-57255 | biostudies-arrayexpress

Comparative gene expression analysis of the human dental pulp in deciduous and permanent teeth

2014-08-21 | GSE51449 | GEO

Characterization of human dental pulp cells grown in chemically defined serum-free medium

Project description:Dental pulp cells obtained from several donors proliferated actively in a serum-free medium STK2. The growth rate of dental pulp cells from most donors was higher in the serum-free medium than that in a medium containing 10% serum. DNA microarray analyses showed that gene expression profile of dental pulp cells grown in the serum-free medium was similar to that of cells grown in a medium containing 10% serum. However, several genes related to cell proliferation were up-regulated in dental pulp cells grown in the serum-free medium.

2018-03-14 | GSE97199 | GEO

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