The histone methyltransferase Setd8 represses Gata2 expression and regulates erythroid maturation

ABSTRACT: The chromatin modifying enzymes that drive the erythroid-specific transcription program are incompletely understood. Setd8 is the sole histone methyltransferase in mammals capable of generating mono-methylated histone H4 lysine 20 (H4K20me1) and is expressed at significantly higher levels in erythroid cells than any other cell- or tissue- type, suggesting that Setd8 has an erythroid-specific function. To test this hypothesis, stable knockdown of Setd8 was established in extensively self-renewing erythroblasts (ESREs), a well-characterized, non-transformed, model of erythroid maturation. Setd8 knockdown impaired erythroid maturation, characterized by a delay in hemoglobin accumulation, larger cell area, persistent kit expression, incomplete nuclear condensation, and lower rates of enucleati

ORGANISM(S): Mus musculus

SUBMITTER: Laurie Steiner 

PROVIDER: E-GEOD-62647 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress