Gene expression of human blood Natural Killer (NK) cells directly after isolation and after manual or automated ex vivo expansion

ABSTRACT: Ex vivo activation and expansion of natural killer (NK) cells is a strategy to produce sufficient numbers of these effector cells for adoptive immunotherapy. Therefore we aimed at the development of an automated, clinical scale NK cell expansion process and compared NK cells after manual and automated expansion.We found only a small subset of 124 genes that significantly varied between both sample groups. These genes were associated with movement of leukocytes were identified including a group of genes for NK cell movement (CMKLR1, CX3CR1, S1PR5, GNLY and CXCR1) which were slightly higher expressed after automated compared to manual expansion. Nevertheless, the expansion profiles of NK cells after automated or manual expansion were rather similar in comparison to the remarkable difference

ORGANISM(S): Homo sapiens

SUBMITTER: Jutta Kollet 

PROVIDER: E-GEOD-62654 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress