Comprehensive profiling of amino acid response uncovers unique methionine-deprived response dependent on intact creatine biosynthesis

ABSTRACT: Besides being building blocks for protein synthesis, amino acids serve a wide variety of cellular functions, including acting as metabolic intermediates for ATP generation and for redox homeostasis. Upon amino acid deprivation, free uncharged tRNAs trigger GCN2-ATF4 to mediate the well-characterized transcriptional amino acid response (AAR). However, it is not clear whether the deprivation of different individual amino acids triggers identical or distinct AARs. Here, we characterized the global transcriptional response upon deprivation of one amino acid at a time. With the exception of glycine, which was not required for the proliferation of MCF7 cells, we found that the deprivation of most amino acids triggered a shared transcriptional response that included the activation of ATF4, p53 an

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaohu Tang 

PROVIDER: E-GEOD-62673 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress