Maternal high-fat diet and obesity compromise fetal hematopoiesis

ABSTRACT: Objective Recent evidence indicates that the adult hematopoietic system is susceptible to diet-induced lineage skewing. It is not known whether the developing hematopoietic system is subject to metabolic programming via in utero high fat diet (HFD) exposure, an established mechanism of adult disease in several organ systems. We previously reported substantial losses in offspring liver size with prenatal HFD. As the liver is the main hematopoietic organ in the fetus, we asked whether the developmental expansion of the hematopoietic stem and progenitor cell (HSPC) pool is compromised by prenatal HFD and/or maternal obesity. Methods We used quantitative assays, progenitor colony formation, flow cytometry, transplantation, and gene expression assays with a series of dietary manipulations

ORGANISM(S): Mus musculus

SUBMITTER: Sophia Jeng 

PROVIDER: E-GEOD-62715 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress