C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria

ABSTRACT: RNA interference (RNAi) is a phylogenetically widespread gene silencing process triggered by doublestranded RNA (dsRNA). In plants and C. elegans, two distinct populations of small RNAs have been proposed to participate in RNAi : "Primary siRNAs" (derived from Dicer nuclease-mediated cleavage of the original trigger) and "Secondary siRNAs" (additional small RNAs whose synthesis requires an RNA-directed RNA polymerase [RdRP]). Analyzing small RNAs associated with ongoing RNAi in C. elegans, we found secondary siRNAs to comprise the vast majority. The bulk of secondary siRNAs exhibited structure and sequence indicative of a biosynthetic mode where each molecule derives from an independent de novo initiation by RdRP. Analysis of endogenous small RNAs indicated that a fract

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Andrew Fire 

PROVIDER: E-GEOD-6282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress