Early reprogramming stages and regulators identified by prospective isolation and single cell mass cytometry

ABSTRACT: In the context of most induced pluripotent stem (iPS) cell reprogramming methods, heterogeneous populations of nonproductive and staggered productive intermediates arise at different reprogramming time points1-11. Despite recent reports claiming substantially increased reprogramming efficiencies using genetically modified donor cells12,13 prospectively isolating distinct reprogramming intermediates remains an important goal to decipher reprogramming mechanisms. Previous attempts to identify surface markers of intermediate cell populations were based on the assumption that during reprogramming the cells progressively lose donor cell identity and gradually acquire iPS cell properties1,2,7,8,10. Here, we report that iPS cell and epithelial markers, such as SSEA1 and EpCAM, respectively, are

ORGANISM(S): Mus musculus

SUBMITTER: Marius Wernig 

PROVIDER: E-GEOD-62957 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress