Enhancing the Functional Maturity of iPSC-Derived Human Hepatocytes Via Controlled Presentation of Cell-Cell Interactions In Vitro

ABSTRACT: Induced pluripotent stem cell-derived human hepatocyte-like cells (iHeps) could provide a powerful tool for studying the mechanisms underlying human liver development and disease, testing the efficacy and safety of pharmaceuticals across different patients (i.e. personalized medicine), and enabling cell-based therapies in the clinic. However, current in vitro protocols that rely upon growth factors and extracellular matrices (ECM) alone yield iHeps with low levels of liver functions relative to adult primary human hepatocytes (PHHs). Moreover, these low hepatic functions in iHeps are difficult to maintain for prolonged times (weeks to months) in culture. Here, we engineered a micropatterned co-culture (iMPCC) platform in a multi-well format that, in contrast to conventional confluent cultu

ORGANISM(S): Homo sapiens

SUBMITTER: Salman Khetani 

PROVIDER: E-GEOD-62962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress