Analysis of allele specific expression and its chromatin state to identify genes that are escaping X chromosome inactivation

ABSTRACT: Disappearance of the Barr body has long been considered a hallmark of cancer, although whether this corresponds to epigenetic instability and transcriptional reactivation, or to genetic loss, has remained unclear. Here we show that in breast cancer cell lines as well as primary breast tumors, the inactive X chromosome frequently displays a highly abnormal 3D nuclear organization, with global perturbations in its characteristic heterochromatic state, including apparent gain of euchromatic marks and lessening of repressive marks such as H3K27me3 and promoter DNA methylation. Genome-wide profiling of chromatin and transcription reveal modified epigenomic landscapes in cancer cells accompanied by a significant degree of X-linked gene reactivation, affecting genes previously implicated in cance

ORGANISM(S): Homo sapiens

SUBMITTER: Ronan ChalignM-CM-) 

PROVIDER: E-GEOD-62966 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress