Comparison of gene expression profile of human trabecular meshwork cells induced by triamcinolone with dexamethasone

ABSTRACT: PURPOSE. Triamcinolone acetonide (TA) and dexamethasone (DEX) are corticosteroids commonly used for ocular inflammation but both can cause ocular hypertension. We investigated the differential gene expression profile of human trabecular meshwork (TM) cells in response to treatment by TA compared to that by DEX. METHODS. Total RNA was extracted from cultured human TM cells treated with TA or DEX and used for microarray gene expression analysis. The microarray experiments were repeated three times. Differentially expressed genes were identified by an empirical Bayes approach and confirmed by real-time quantitative PCR. RESULTS. 0.1 mg/ml TA treatment resulted in 15 genes up-regulated and 12 genes down-regulated while 1mg/ml TA treatment resulted in 36 genes up-regulated and 21 genes down-reg

ORGANISM(S): Homo sapiens

SUBMITTER: Bao Jian Fan 

PROVIDER: E-GEOD-6298 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress