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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of white and grey matter in the distribution on the middle cerebral artery 6 hours after administration of the saline/catalytic antioxidant AEOL 10150 and ischemia/reperfusion of the MCAO to study the effect of AEOL 10150 on gene expression.

ABSTRACT: A catalytic antioxidant (AEOL 10150) attenuates expression of inflammatory genes in stroke. White and grey matter in the distribution on the middle cerebral artery 6 hours after administration of the saline/catalytic antioxidant AEOL 10150 and ischemia/reperfusion of the MCAO; pooled mRNA from 6 brains.

ORGANISM(S): Mus musculus

SUBMITTER: Russell Bowler

PROVIDER: E-GEOD-63 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A catalytic antioxidant (AEOL 10150) attenuates expression of inflammatory genes in stroke.

Bowler Russell P RP Sheng Huaxin H Enghild Jan J JJ Pearlstein Robert D RD Warner David S DS Crapo James D JD

Free radical biology & medicine 20021001 8

Oxidative stress is a major source of injury from cerebral ischemia and reperfusion. We hypothesized that a catalytic antioxidant AEOL 10150 [manganese (III) meso-tetrakis (di-N-ethylimidazole) porphyrin] would attenuate changes in brain gene expression in a mouse model of transient middle cerebral artery occlusion (MCAO). C57BL/6J mice were subjected to either sham surgery or 60 min of right MCAO. AEOL 10150 or phosphate-buffered saline was given intravenously 5 min after onset of reperfusion ( ...[more]

PMID: 12374626

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