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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Zeb1 knockdown NM18 cells

ABSTRACT: Differentiation of epithelial cells is strongly affected by transcription factors related to epithelial to mesenchymal-like progression. We used gene expression profiling to identify genes that are differentially regulated upon repression of the transcription factor Zeb1 (deltaEF1/Areb6). NM18 cells were transfected with non-silencing siRNA (ns-siRNA) or siRNA targeting Zeb1 (Zeb1 siRNA) for 48h. Experiments were performed as independent biological triplicate. Three non-silencing siRNA samples (GSM1322708, GSM1322709, and GSM1322710) were originally deposited in Series GSE54715. The samples were reanalyzed with the Zeb1 siRNA samples and the data is linked at the bottom of the Series record.

ORGANISM(S): Mus musculus

SUBMITTER: Alexander Feuerborn

PROVIDER: E-GEOD-63164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Zeb1 affects epithelial cell adhesion by diverting glycosphingolipid metabolism.

Mathow Daniel D Chessa Federica F Rabionet Mariona M Kaden Sylvia S Jennemann Richard R Sandhoff Roger R Gröne Hermann-Josef HJ Feuerborn Alexander A

EMBO reports 20150202 3

This study proposes that the transcription factor Zeb1 modulates epithelial cell adhesion by diverting glycosphingolipid metabolism. Zeb1 promotes expression of a-series glycosphingolipids via regulating expression of GM3 synthase (St3gal5), which mechanistically involves Zeb1 binding to the St3gal5 promoter as well as suppressing microRNA-mediated repression of St3gal5. Functionally, the repression of St3gal5 suffices to elevate intercellular adhesion and expression of distinct junction-associa ...[more]

PMID: 25643708

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Gene expression analysis of SW480 colorectal cancer cells with siRNA mediated ZEB1 or control (GFP) knockdown

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2016-06-15 | E-MTAB-3479 | biostudies-arrayexpress

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2014-03-01 | E-GEOD-54715 | biostudies-arrayexpress

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Expression data from Zeb1 knockdown NM18 cells

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2015-02-04 | GSE63164 | GEO

RNA-seq of human colorectal cancer cells in presence and absence of ZEB1

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2023-07-31 | GSE123416 | GEO

Novel strategies to enforce an epithelial phenotype in mesenchymal cells

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2014-05-19 | E-GEOD-57660 | biostudies-arrayexpress

Defining the role of ZEB1 in the pathogenesis of lung cancer

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2016-08-31 | GSE77925 | GEO

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