Deep Sequencing-Based Transcriptome Analysis of Chicken Spleen in Response to J Subgroup Avian Leukosis Virus (ALV-J) Infection.

ABSTRACT: Purpose: The goals of this study are to investigate the differentially expressed genes between ALV-J infected (WRR+) and uninfected (WRR-)chickens spleens by Illumina deep sequencing. Methods: 140-day-old female chickens of White Recessive Rock (WRR) were confirmed as J subgroup avian leukosis virus (ALV-J) infection. Total RNA from three ALV-J-infected spleens (designated: WRR1+, WRR2+, WRR3+) and three uninfected normal spleen samples (designated: WRR1-, WRR2-, WRR3-) was isolated by TRIzol following the manufacturer’s instruction (Invitrogen, CA, USA). RNA samples of three individuals within each group were pooled with equal amounts, and then were subjected to Illumina deep sequencing by Illumina Genome Analyzer IIx. Results: Through raw data processed, 49,979,648 and 43,704,401 clean r

ORGANISM(S): Gallus gallus

SUBMITTER: Zhenhui Li 

PROVIDER: E-GEOD-63226 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress