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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Real-time quantitative PCR analysis of 3T3-L1 cells treated with Interleukin-15 (IL-15)

ABSTRACT: Differentiated 3T3-L1 adipocytes were either treated 0ng/ml or 500ng/ml rmIL-15 for 24 hours (n=3/group) qPCR gene expression profilling performed in vitro using recombinant murine (rm)IL-15 treated (500ng/ml) and untreated (control) 3T3-L1 cells. Equal amounts of total RNA per subject were used in the assay.

ORGANISM(S): Mus musculus

SUBMITTER: Ali Ashkar

PROVIDER: E-GEOD-63253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Real-time quantitative PCR analysis of 3T3-L1 cells treated with Interleukin-15 (IL-15)

Project description:Differentiated 3T3-L1 adipocytes were either treated 0ng/ml or 500ng/ml rmIL-15 for 24 hours (n=3/group)

2014-11-14 | GSE63253 | GEO

MicroRNA profiling of mouse 3T3-L1 cells before and after adipogenesis

Project description:Compare miRNA expression profiles between undifferentiated 3T3-L1 preadipocytes (Day 0) and differentiated 3T3-L1 adipocytes (Day 9)

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Detection of microRNA expression during 3T3-L1 pre-adipocyte differentiation using a custom made microarray chip.

Project description:Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of events including clonal expansion, growth arrest and terminal differentiation. The mechanisms coordinating these different steps are not yet fully understood. Here we investigated whether micro (mi)RNAs play a role in this process. Microarray analysis was performed to detect miRNA expression during 3T3-L1 preadipocyte differentiation. Several miRNAs, including let-7, were up-regulated during 3T3-L1 adipogenesis. Ectopic introduction of let-7 into 3T3-L1 cells inhibited clonal expansion as well as terminal differentiation. The mRNA encoding high mobility group AT-hook 2 (HMGA2), a transcription factor that regulates growth and proliferation in other contexts, was inversely correlated with let-7 levels during 3T3-L1 cell adipogenesis, and let-7 markedly reduced HMGA2 concentrations. Knockdown of HMGA2 inhibited 3T3-L1 differentiation. These results suggest that let-7 plays an important role in adipocyte differentiation and that it does so in part by targeting HMGA2, thereby regulating the transition from clonal expansion to terminal differentiation. 3T3-L1 cells were induced to differentiation into mature adipocytes using a canonical DMI cocktail. The time point at two days after confluency of 3T3-L1 was defined as day 0. Samples were collected at day 0, day 1, day 4, and day 7. The expression of microRNAs at day 1, day 4, and day 7 was compared to that of day 0.

2010-05-18 | E-GEOD-16229 | biostudies-arrayexpress

Transcription profiling of mouse 3T3-L1 adipocytes vs. adipocyte-derived microvesicles (ADMs)

Project description:mRNA profiles of adipocyte-derived microvesicles (ADMs) and their donor 3T3-L1 adipoyctes (Day 11) were compared. ADMs included RNA without typical 28S and 18S ribosomal RNA. Experiment Overall Design: ADMs vs. donor 3T3-L1 adipocytes. Biological replicates: 2 ADMs replicates, 2 donor cells.

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Importance of histone demethylation in adipogenic differentiation and function

Project description:This SuperSeries is composed of the following subset Series: GSE18598: Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine GSE18599: Differentiating 3T3-L1 adipocytes, introduced with siRNA against phf21a gene Refer to individual Series

2012-04-04 | E-GEOD-18600 | biostudies-arrayexpress

Differentiating 3T3-L1 adipocytes, introduced with siRNA against phf21a gene

Project description:Adipogenic differentiation and metabolic adaptation are initiated through transcriptional and epigenetic reprogramming. In particular, dynamic changes in histone modifications may play central roles in the rearrangement of gene expression patterns. BHC80 protein, encoded by phf21a gene, is a part of LSD1 histone demethylase complex and is essential for the demethylation activity. To address the importance of histone demethylation in adipogenic differentiation and function, we performed cDNA microarray in BHC80-deficient 3T3-L1 cells 3T3-L1 preadipocytes were transfected with either BHC80-specific siRNA or control siRNA (siGL3). 24 hours later, cells were subjected to adipogenic induction. 24 hours later, cells were harvested for total RNA extraction.

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Project description:To identify and elucidate the mechanisms of adipogenic differentiation within the mouse 3t3-l1 cell line

2019-11-26 | E-MTAB-8200 | biostudies-arrayexpress

Mus musculus

Project description:3T3-L1-NC V.S 3T3-L1-LIGHT RNA-seq

| PRJNA451383 | ENA

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Project description:Cebpa is a critical transcription factor gene for adipocyte differentiation and adipose tissue development. However, mechanisms controlling Cebpa expression during adipogenic differentiation remain largely unknown. Here, we generated the high-resolution chromatin interaction maps of Cebpa in 3T3-L1 preadipocytes (3T3-L1) and 3T3-L1 adipocytes (3T3-L1-AD) using circularized chromosome conformation capture coupled with next-generation sequencing (4C-seq), and characterized differences in their chromatin interactomes and chromatin status of the interaction sites during adipogenic differentiation. We performed a 4C-seq experiment on inguinal white adipose tissue (iWAT) to evaluate whether chromatin interaction between Cebpa-L1-AD-En2 and Cebpa promoters in 3T3-L1 adipocytes also exists in mouse adipose tissue.

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Characterizing the profiles of Ebf1 in mouse fat cells (ChIP-seq)

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