ELAV ChIP-seq in Drosophila melanogaster embryos

ABSTRACT: Alternative polyadenylation (APA) has been implicated in a variety of developmental and disease processes, such as stem cell differentiation and cancer. A particularly dramatic form of APA has been documented in the developing nervous system of flies and mammals, whereby a variety of neurogenic genes undergo coordinate extension of their 3’ UTRs. In Drosophila, the RNA-binding protein ELAV inhibits RNA processing at proximal polyadenylation (poly(A)) sites, thereby fostering the formation of 3’ extensions that can reach 12 kb in length. Here, we present evidence that paused Pol II plays an important role in the selective recruitment of ELAV to elongated genes. Replacing native promoters of elongated genes with heterologous promoters blocks normal 3’ extension in the nervous system, while n

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Wei Zhang 

PROVIDER: E-GEOD-63323 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress