Transcriptional effects of glucocorticoid receptor alpha and beta signaling in zebrafish

ABSTRACT: The glucocorticoid receptor (GR) regulates gene expression upon activation by glucocorticoid (GC) hormones. In zebrafish, two GR splice variants exist: the canonical GR M-NM-1-isoform (GRM-NM-1), and the GR M-NM-2-isoform (GRM-NM-2). The exact function of GRb remains elusive. We have investigated the transcriptional role of GRa and GRb in the zebrafish embryo model by injecting mebryos with two splice-blocking morpholinos (one leading to knockdown of both GR M-NM-1- and M-NM-2-isoforms, and another targeting the alternative splicing of the GR pre-mRNA in favor of the GR M-NM-2-isoform) and with GRM-NM-2 mRNA (resulting in specific GRM-NM-2 overexpression). Transcriptome profiling was performed on total RNA isolated from 30 hpf embryos. Our results show that GRb does not act as a dominant-n

ORGANISM(S): Danio rerio

SUBMITTER: Marcel Schaaf 

PROVIDER: E-GEOD-63360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress