The RNA binding protein Arrest (Aret) regulates myofibril maturation in Drosophila flight muscle

ABSTRACT: In Drosophila, fibrillar flight muscles (IFMs) enable flight, while tubular muscles mediate other body movements. Here, we use RNA-sequencing and isoform-specific reporters to show that spalt major (salm) determines fibrillar muscle physiology by regulating transcription and alternative splicing of a large set of sarcomeric proteins. We identify the RNA binding protein Arrest (Aret, Bruno) as downstream of salm. Aret shuttles between cytoplasm and nuclei, and is essential for myofibril maturation and sarcomere growth of IFMs. Molecularly, Aret regulates IFM-specific transcription and splicing of various sarcomeric targets, including Stretchin and wupA (TnI), and thus maintains muscle fiber integrity. As Aret and its sarcomeric targets are evolutionarily conserved, similar principles may re

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Maria Spletter 

PROVIDER: E-GEOD-63707 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress