Expression analysis of wild-type and Tfam heterozygous knockout (Tfam+/-) murine embryonic fibroblasts (MEFs).

ABSTRACT: The goal of this analysis was to utilize microarray profiling to identify basal alterations in gene expression in response to TFAM depletion and mtDNA stress. Mitochondrial DNA (mtDNA) is normally present at thousands of copies per cell and is packaged into several hundred higher-order structures termed nucleoids. The abundant mtDNA-binding protein, TFAM (transcription factor A,mitochondrial), regulates nucleoid architecture, abundance and segregation. Complete mtDNA depletion profoundly impairs oxidative phosphorylation, triggering calcium-dependent stress signalling and adaptive metabolic responses. However, the cellular responses to mtDNA instability, a physiologically relevant stress observed in many human diseases and ageing, remain poorly defined. Here we show that moderate mtDNA str

ORGANISM(S): Mus musculus

SUBMITTER: Phillip West 

PROVIDER: E-GEOD-63767 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress