Functional screen for novel regulators of murine hematopoietic stem cell in vivo repopulating activity

ABSTRACT: Although Hematopoietic Stem Cell Transplantation (HSCT) routinely treats hematologic disease, many patients experience adverse outcomes. Understanding the molecular regulation of HSC engraftment is paramount to improving HSCT regimens. Here, we executed a large-scale transplant-based functional screen for novel regulators of HSC repopulation.. Of >50 gene candidates tested, 18 were required for in vivo hematopoietic repopulation and two were detrimental to repopulation, as their loss enhanced this activity. Each Hit was validated in a second screen. Eleven Hits have never before been implicated in HSC biology. We further show that one novel Hit, Foxa3, is required for optimal engraftment as Foxa3-/- bone marrow is defective in both primary and secondary hematopoietic reconstitution.

ORGANISM(S): Mus musculus

SUBMITTER: David Finkelstein 

PROVIDER: E-GEOD-63830 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress