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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression changes in Paxillus involutus- Betula pendula ectomycorrhizal roots compared to Paxillus involutus mycelium patches

ABSTRACT: Illumina HiSeq technology was used to generate mRNA profiles from Paxillus involutus ectomycorrhizal roots compared to mycelium patches . Mycorrhizal roots were harvested after 4 weeks, pooled and used for RNA extraction. Reads of 2X100bp were generated and aligned to Paxillus involutus (http://genome.jgi-psf.org/Paxin1/Paxin1.home.html) using CLC Genomics Workbench 6. mRNA profiles from Paxillus involutus ectomycorrhizal roots and mycelium patches were generated by paired-end (2x100bp) Illumina HiSeq2000 sequencing. Two biological replicates were sequenced for mycorrhizal and mycelium samples.

ORGANISM(S): Paxillus involutus

SUBMITTER: Annegret Kohler

PROVIDER: E-GEOD-63924 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression changes in Paxillus involutus- Betula pendula ectomycorrhizal roots compared to Paxillus involutus mycelium patches

Project description:Illumina HiSeq technology was used to generate mRNA profiles from Paxillus involutus ectomycorrhizal roots compared to mycelium patches . Mycorrhizal roots were harvested after 4 weeks, pooled and used for RNA extraction. Reads of 2X100bp were generated and aligned to Paxillus involutus (http://genome.jgi-psf.org/Paxin1/Paxin1.home.html) using CLC Genomics Workbench 6.

2015-02-06 | GSE63924 | GEO

Transcription profiling of different tissues of the ECM interaction between Betula pendula and Paxillus involutus grown in peat microcosms containing (NH4)2SO4 nutrient patches

Project description:A microarray analysis was conducted to analyze gene expression patterns in different tissues of the ECM interaction between Betula pendula and Paxillus involutus grown in peat microcosms containing (NH4)2SO4 nutrient patches. Expression profiles for mycorrhizal root tips (TIP), rhizomorphs (CORD) and the mycelium harvested from (NH4)2SO4 nutrient patches (PATCH) were compared. Our goal was to identify genes that showed nutrient- and tissue-specific differential regulation. <br> <br> The entire design involved 9 slides (DW1_01--DW1_09), 12 labelled extracts, and 3 biological replicates (R1-R3). The experiment was designed as a simple loop (all-pairwise comparisons) of the three tissues including dye-swap, biological and technical replication.

2005-08-01 | E-MEXP-275 | biostudies-arrayexpress

Comparative genomic hybridization of Paxillus involutus strains

Project description:Variation in gene content in strains from genetically isolated lineages in Paxillus involutus

2009-10-01 | E-MEXP-1499 | biostudies-arrayexpress

Transcription profiling of ectomycorrhizal fungus Paxillus involutus grown with different sources of nitrogen representing various degrees of complexity

Project description:A microarray analysis to investigate transcriptional responses in the ectomycorrhizal fungus Paxillus involutus, after providing various sources of nitrogen. Five different sources of nitrogen representing various degrees of complexity were provided as patches in peat microcosms for fungal ingrowth: ammonium phosphate (APO), ammonium sulphate (ASU), glutamine (GLN), bovine serum albumin (BSA), and chitin (CHI). After fungal establishment of patches total RNA was isolated and used for global transcriptional analyses using cDNA microarrays. The entire design involved 9 slides (in the range DW2_01--DW2_13), 14 labelled extracts, and 3 biological replicates (R1-R3). The experiment was designed as a loop combining the 5 diffrent samples and including dye-swaps and biological replication. COMMENT: In our analysis of the raw dataset, 3 channels were removed due to poor signal quality: array 12707648b, Cy5 (635nm) channel (GLN R1 sample); array 12707650c, Cy5 (635nm) channel (APO R1 sample); array 12707651b, Cy5 (635nm) channel (APO R1 sample).

2008-06-01 | E-MEXP-1257 | biostudies-arrayexpress

Expression analysis from glass-bead cultures of Paxillus involutus (ATCC200175) after amendments of 3 different substrates

Project description:Abstract: Proteins contribute to a major part of the organic nitrogen in forest soils. This nitrogen is mobilized and becomes available to trees as a result of the depolymerizing activities of symbiotic ectomycorrhizal fungi. The mechanisms by which these fungi depolymerize proteins and assimilate the released nitrogen are poorly characterized. Biochemical analysis and transcriptome profiling were performed to examine the proteolytic machinery and the uptake system of the ectomycorrhizal basidiomycete Paxillus involutus during the assimilation of organic nitrogen from various protein sources and extracts of organic matter. All substrates induced secretion of peptidase activity with an acidic pH optimum, mostly contributed by aspartic peptidases. The peptidase activity was transiently repressed by ammonium. Transcriptional analysis revealed a large number of extracellular endo- and exopeptidases. The expression levels of these peptidases were regulated in parallel with transporters and enzymes involved in the assimilation and metabolism of the released peptides and amino acids. For the first time the molecular components of the protein degradation pathways of an ectomycorrhizal fungus are described. The data suggest that the transcripts encoding these components are regulated in response to the chemical properties and the availability of the protein substrates. Firoz Shah, Francois Rineau, Björn Canbäck, Tomas Johansson and Anders Tunlid (2013) The molecular components of the extracellular protein-degradation pathways of the ectomycorrhizal fungus Paxillus involutus (submitted) A one-chip study (data from 9 subarrays collected from a 12-plex Nimblegen microarray (ID: 461228)) using total RNA recovered from glass-bead cultures of Paxillus involutus (ATCC200175) after amendments of 3 different substrates (3 replicates of each).

2013-09-01 | E-GEOD-47838 | biostudies-arrayexpress

Expression analysis from glass-bead cultures of Paxillus involutus (ATCC200175) after amendments of nitrogen (+/-NH4) to substrate followed by incubation for different periods of time

Project description:Abstract: Proteins contribute to a major part of the organic nitrogen in forest soils. This nitrogen is mobilized and becomes available to trees as a result of the depolymerizing activities of symbiotic ectomycorrhizal fungi. The mechanisms by which these fungi depolymerize proteins and assimilate the released nitrogen are poorly characterized. Biochemical analysis and transcriptome profiling were performed to examine the proteolytic machinery and the uptake system of the ectomycorrhizal basidiomycete Paxillus involutus during the assimilation of organic nitrogen from various protein sources and extracts of organic matter. All substrates induced secretion of peptidase activity with an acidic pH optimum, mostly contributed by aspartic peptidases. The peptidase activity was transiently repressed by ammonium. Transcriptional analysis revealed a large number of extracellular endo- and exopeptidases. The expression levels of these peptidases were regulated in parallel with transporters and enzymes involved in the assimilation and metabolism of the released peptides and amino acids. For the first time the molecular components of the protein degradation pathways of an ectomycorrhizal fungus are described. The data suggest that the transcripts encoding these components are regulated in response to the chemical properties and the availability of the protein substrates. Firoz Shah, Francois Rineau, Björn Canbäck, Tomas Johansson and Anders Tunlid (2013) The molecular components of the extracellular protein-degradation pathways of the ectomycorrhizal fungus Paxillus involutus (submitted) A one-chip study (data from 12 subarrays collected from 12-plex Nimblegen microarray (IDs: 527896) using total RNA recovered from glass-bead cultures of Paxillus involutus (ATCC200175) after amendments of nitrogen (+/-NH4) to substrate followed by incubation for different periods of time (3 replicates of each).

2013-09-01 | E-GEOD-47839 | biostudies-arrayexpress

Paxillus involutus

Project description:Gene expression changes in Paxillus involutus- Betula pendula ectomycorrhizal roots compared to Paxillus involutus mycelium patches

| PRJNA269501 | ENA

Transcription profiling of Populus x canescens fine roots inoculated with Paxillus involutus in response to salt stress

Project description:Populus x canescens was inoculated with Paxillus involutus and grown in a climate chamber for 13 weeks. Afterwards, plants were salt stressed for 18 additional days with 150 mM NaCl in the nutrient solution. This resulted in 4 different treatments: no mycorrhiza<br>control (NC), mycorrhiza/control (MC), no mycorrhiza/salt (NS), mycorrhiza/salt (MS).

2009-09-16 | E-MEXP-1874 | biostudies-arrayexpress

Comparative genomic hybridization of 5 strains of Paxillus filamentosus and 1 strain of Paxillus involutus

Project description:Variations in gene content and sequence that could be associated with symbiotic adaptations of the ectomycorrhizal fungus Paxillus involutus were investigated by analyses of strains showing various abilities to form mycorrhiza. Five strains of Paxillus involutus (ATCC 200175, Maj; Nau, Pi01SE, and Pi08BE) and one strain of Paxillus filamentosus (Pf01De) were analyzed by comparative genomic hybridizations using cDNA microarrays. Two batches of arrays were used containing 1,076 unique fungal reporters. DNA was prepared from each strain, and after fragmentation and labelling used for dual-label microarray hybridizations. The experimental design includes 16 arrays (CGH_01 -- CGH_16), of which 12 arrays represent dye-swapped and direct contrasts between the sample strains and the reference strain ATCC 200175. Two arrays represent dye-swapped self-self hybridizations of the reference strain ATCC 200175 (CGH_01 and CGH_02). The remaining two arrays represent dye-swapped and direct contrasts between the sample strains Maj and Nau (CGH_06 and CGH_07).

2006-01-01 | E-MEXP-437 | biostudies-arrayexpress

Gene expression changes in Piloderma croceum-Quercus robur ectomycorrhizal roots compared to Piloderma croceum free-living mycelium

Project description:Illumina HiSeq technology was used to generate mRNA profiles from Piloderma croceum ectomycorrhizal roots compared to free-living mycelium . Mycorrhizal roots were harvested after 8 weeks, pooled and used for RNA extraction. Reads of 2X100bp were generated and aligned to Piloderma croceum (http://genome.jgi-psf.org/Pilcr1/Pilcr1.home.html) using CLC Genomics Workbench 6. mRNA profiles from Piloderma croceum ectomycorrhizal roots and free-living mycelium were generated by paired-end (2x100bp) Illumina HiSeq2000 sequencing. Three biological replicates were sequenced for mycorrhizal and mycelium samples.

2015-02-06 | E-GEOD-63925 | biostudies-arrayexpress

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