Changes in liver gene expression profiles during sexual maturation of European eel (Anguilla anguilla) males.

ABSTRACT: Gene expression analyses have been performed on liver tissue of sexually mature and immature males using microarrays. 60 eels were transferred to two independent temperature controlled recirculation water units connected to two 500 L cylindro-conical tanks (30 fish per tank) where the fish were acclimated to seawater (35 PSU salinity) over a 2 week period.Eel males in one of the seawater units were injected intramuscularly every week over a 140 day period with 2000 IU hCG/kg (human chorionic gonadotropin, Sigma–Aldrich Chemical) dissolved in 0.9% saline to induce sexual maturation. Eel males in the other recirculation unit were injected weekly over the same period with 0.9% NaCl (vehicle).At the end of the experiment, eels were anesthetized in a solution of 0.1mg/L tricaine methanesulfonat

ORGANISM(S): Anguilla anguilla

SUBMITTER: Massimo Milan 

PROVIDER: E-GEOD-64067 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress