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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data of leaves from transgenic barley expressing wheat Lr34 gene

ABSTRACT: The wheat gene Lr34 (Yr18/Pm38/Sr57/Ltn1) encodes a putative ABCG-type of transporter and is a unique source of disease resistance providing durable and partial resistance against multiple fungal pathogens. Lr34 has been found to be functional as a transgene in barley. We used microarrays to decipher the changes in global gene expression governed by Lr34 expression in barley. Leaves from two independent lines of transgenic barley plants were selected at seedling (two weeks old plants) and mature (six weeks old plants) stages of development for RNA extraction and hybridization on Affymetrix microarrays. We used three biological replicates for expression profiles. Wild type plants served as controls.

ORGANISM(S): Hordeum vulgare

SUBMITTER: Harsh Chauhan

PROVIDER: E-GEOD-64228 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The wheat resistance gene Lr34 results in the constitutive induction of multiple defense pathways in transgenic barley.

Chauhan Harsh H Boni Rainer R Bucher Rahel R Kuhn Benjamin B Buchmann Gabriele G Sucher Justine J Selter Liselotte L LL Hensel Goetz G Kumlehn Jochen J Bigler Laurent L Glauser Gaëtan G Wicker Thomas T Krattinger Simon G SG Keller Beat B

The Plant journal : for cell and molecular biology 20151001 1

The wheat gene Lr34 encodes an ABCG-type transporter which provides durable resistance against multiple pathogens. Lr34 is functional as a transgene in barley, but its mode of action has remained largely unknown both in wheat and barley. Here we studied gene expression in uninfected barley lines transgenic for Lr34. Genes from multiple defense pathways contributing to basal and inducible disease resistance were constitutively active in seedlings and mature leaves. In addition, the hormones jasmo ...[more]

PMID: 26315512

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