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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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High-throughput RNA sequencing of methanosarcina grown on methylated sulfur compounds

ABSTRACT: Methylated sulfur compounds, including dimethylsulfide (DMS), methylmercaptopropionic acid (MMPA) and methylsulfide (MeSH), are well-documented to play roles in global sulfur cycle and climate homeostasis, yet the molecular mechanisms of how they are metabolized by methanogens remain largely uncharacterized. Here, using high-throughtput sequencing of RNA (RNA-seq), we gained insight into how methanogens respond to methylated sulfur compounds at the transcriptional level. The mRNA from wild-type of Methanosarcina acetivorans C2A grown on methylated sulfur compounds were harvested, sequenced and mapped to the genome. Then, we compared RNA-seq profiles to that grown on MeOH in search of unque genes.

ORGANISM(S): Methanosarcina acetivorans C2A

SUBMITTER: William Metcalf

PROVIDER: E-GEOD-64349 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic basis for metabolism of methylated sulfur compounds in Methanosarcina species.

Fu He H Metcalf William W WW

Journal of bacteriology 20150217 8

<h4>Unlabelled</h4>Methanosarcina acetivorans uses a variety of methylated sulfur compounds as carbon and energy sources. Previous studies implicated the mtsD, mtsF, and mtsH genes in catabolism of dimethylsulfide, but the genes required for use of other methylsulfides have yet to be established. Here, we show that a four-gene locus, designated mtpCAP-msrH, is specifically required for growth on methylmercaptopropionate (MMPA). The mtpC, mtpA, and mtpP genes encode a putative corrinoid protein, ...[more]

PMID: 25691524

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High-throughput RNA sequencing of methanosarcina grown on methylated sulfur compounds

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Methanosarcina acetivorans C2A

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Transcriptome and translatome landscape of Streptomyces coelicolor M145

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