RNA-sequencing based analysis of transcriptomes of Listeria monocytogenes grown on cold smoked salmon and modified BHI broth

ABSTRACT: In this study, RNA-seq was used to compare the transcriptomes of L. monocytogenes strain H7858 grown on cold smoked salmon (CSS) and in modified brain heart infusion broth (MBHIB, water-phase salt 4.65%, pH 6.1) at 7oC. RNA-seq was performed on H7858 RNA samples representing four independent biological replicates of growth experiments that involved growth of H7858 on CSS or in MBHIB. Samples for RNA preparation were collected when H7858 was grown to late log phase on these two matrices. Indexed and purified cDNA libraries (8 libraries including 4 replicates for each CSS and MBHIB) were loaded together onto an independent flow cell without any other samples; sequencing was carried out by running Hiseq 2000 (single-end, 100-bp per read). Reads alignment was carried out using the Burrows-Whe

ORGANISM(S): Listeria monocytogenes

SUBMITTER: Silin Tang 

PROVIDER: E-GEOD-64353 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress