MITF drives endolysosomal biogenesis and potentiates Wnt signaling in melanoma cells

ABSTRACT: Canonical Wnt signaling plays an important role in development and disease, regulating transcription of target genes and stabilizing many proteins phosphorylated by Glycogen Synthase Kinase 3 (GSK3). We observed that the MiT family of transcription factors, which includes the melanoma oncogene MITF and the lysosomal master regulator TFEB, had the highest phylogenetic conservation of three consecutive putative GSK3 phosphorylation sites in animal proteomes. This prompted us to examine the relationship between MITF, endolysosomal biogenesis and Wnt signaling. Here we report that MITF expression levels correlated with the expression of a large subset of lysosomal genes in melanoma cell lines. MITF expression in the Tetracycline-inducible C32 melanoma model caused a marked increase in vesicula

ORGANISM(S): Homo sapiens

SUBMITTER: HSIAOWANG CHEN 

PROVIDER: E-GEOD-64497 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress