Transcription profiling of human neuroblastoma cell line IMR-32 overexpressiong SQSTM1 and DPP3 to identify activators for the antioxidant response element

ABSTRACT: The antioxidant response element (ARE) is a cis-acting regulatory enhancer element found in the 5’ flanking region of many phase II detoxification enzymes. Upregulation of ARE-dependent target genes is known to have neuroprotective effects; yet, the mechanism of activation is largely unknown. By screening an arrayed collection of approximately 15,000 full-length expression cDNAs in the human neuroblastoma cell line IMR-32 with an ARE-luciferase reporter, we have identified several cDNAs not previously associated with ARE activation. A subset of cDNAs, including sequestosome 1 (SQSTM1) and dipeptidylpeptidase III (DPP3), activated the ARE in primary mouse-derived cortical neurons. Overexpression of SQSTM1 and DPP3 in IMR-32 cells stimulated NRF2 nuclear translocation and led to increased le

ORGANISM(S): Homo sapiens

SUBMITTER: John Walker 

PROVIDER: E-GEOD-6451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress