Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [ChIP-seq]

ABSTRACT: MyoD and NeuroD2 are master regulators of myogenesis and neurogenesis and bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage-specification, we generated a MyoD-mutant with the DNA binding specificity of NeuroD2. Our results demonstrate that redirecting MyoD binding from MyoD-private sites to NeuroD2-private sites, despite preserved binding to the MyoD/NeuroD2-shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis. ChIP-seq profiling of MyoD, NeuroD2 and chimera mutants in mouse P19 cells transfected with these genes. The chimeric mutants are MyoD with the bHLH domain replaced with

ORGANISM(S): Mus musculus

SUBMITTER: Zizhen yao 

PROVIDER: E-GEOD-64626 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress