Dynamic response of P. putida S12 and S12M-NM-^TtrgI to sudden addition of toluene

ABSTRACT: Pseudomonas putida S12 is exceptionally tolerant to various organic solvents. To obtain further insight in this bacteriumM-bM-^@M-^Ys primary defence mechanisms towards these potentially harmful substances, we studied its genome wide transcriptional response to sudden addition of toluene. Global gene expression profiles were monitored for 30 minutes after toluene addition. During toluene exposure, high oxygen-affinity cytochrome c oxidase is specifically expressed to provide for an adequate proton gradient supporting solvent efflux mechanisms. Concomitantly, the glyoxylate bypass route was up-regulated, to repair an apparent toluene stress-induced redox imbalance. A knock-out mutant of trgI, a recently identified toluene-repressed gene, was investigated in order to identify TrgI function.

ORGANISM(S): Pseudomonas putida S12

SUBMITTER: Johannes de Winde 

PROVIDER: E-GEOD-64791 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress