Transcription profiling of human MOLT4 cell lines before and after inhibition of gamma secretase to investigate NOTCH signaling in T-cell acute lymphoblastic leukemia cell lines

ABSTRACT: In T-cell acute lymphoblastic leukemia (T-ALL) NOTCH 1 receptors are frequently mutated. This leads to aberrantly high Notch signaling, but how this translates into deregulated cell cycle control and the transformed cell type is poorly understood. In this report, we analyze downstream responses resulting from the high level of NOTCH 1 signaling in T-ALL. Notch activity, measured immediately downstream of the NOTCH 1 receptor, is high, but expression of the canonical downstream Notch response genes HES 1 and HEY 2 is low both in primary cells from T-ALL patients and in T-ALL cell lines. This suggests that other immediate Notch downstream genes are activated, and we found that Notch signaling controls the levels of expression of the E3 ubiquitin ligase SKP2 and its target protein p27Kip1. We

ORGANISM(S): Homo sapiens

SUBMITTER: David Brodin 

PROVIDER: E-GEOD-6495 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress