Human induced pluripotent stem cells generated from intervertebral disc cells improve neurologic functions in spinal cord injury

ABSTRACT: We generated iPSCs from human intervertebral disc cells which were obtained during spine fusion surgery of patients with spinal cord injury. The disc cell-derived iPSCs (diPSCs) showed similar characteristics to human embryonic stem cells (hESCs) and were efficiently differentiated into neural progenitor cells (NPCs) with the capability of differentiation into mature neurons in vitro. To examine whether the transplantation of NPCs derived from the diPSCs showed therapeutic effects, the NPCs were transplanted into mice at 9 days post-spinal cord injury. We detected a significant amelioration of hind limb dysfunction during the follow up recovery periods. Histological analysis at 5 weeks post-transplantation, we could identify undifferentiated human NPCs (Nestin+) as well as early (TUJ1+

ORGANISM(S): Homo sapiens

SUBMITTER: Kangin lee 

PROVIDER: E-GEOD-64964 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress