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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome analysis of wild type and erythromycin-producing strain of S. erythraea

ABSTRACT: Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. We used transcriptomic approach to compare whole genome expression in erythromycin high-producing strain, compared to the wild type S. erythraea strain in four stages of fermentation. 2 strains (3 individual fermentations each), 4 time points --> 24 samples (2 exluded from anaysis, 22 remaining); one color design

ORGANISM(S): Saccharopolyspora erythraea

SUBMITTER: Spela Baebler

PROVIDER: E-GEOD-65007 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea.

Karničar Katarina K Drobnak Igor I Petek Marko M Magdevska Vasilka V Horvat Jaka J Vidmar Robert R Baebler Špela Š Rotter Ana A Jamnik Polona P Fujs Štefan Š Turk Boris B Fonovič Marko M Gruden Kristina K Kosec Gregor G Petković Hrvoje H

Microbial cell factories 20160603

<h4>Background</h4>Omics approaches have significantly increased our understanding of biological systems. However, they have had limited success in explaining the dramatically increased productivity of commercially important natural products by industrial high-producing strains, such as the erythromycin-producing actinomycete Saccharopolyspora erythraea. Further yield increase is of great importance but requires a better understanding of the underlying physiological processes.<h4>Results</h4>To ...[more]

PMID: 27255285

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Transcriptome analysis of wild type and erythromycin-producing strain of S. erythraea

Project description:Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. We used transcriptomic approach to compare whole genome expression in erythromycin high-producing strain, compared to the wild type S. erythraea strain in four stages of fermentation.

2016-06-22 | GSE65007 | GEO

Phenotype and expression profile of S. erythraea rifampicin-resistant (rif) mutants affected in erythromycin production

Project description:In this study we have used the rifampicin selection as a tool to genetically improve the erythromycin producer Saccharopolyspora erythraea. Two rifampicin-resistant (rif) mutants, rif1 and rif6, have been characterized in more detail. With respect to the parental strain NRRL2338, rif1 (harboring the missense S444F) exhibited higher respiratory performance and final erythromycin yields; in contrast, rif6 (harboring the missense Q426R) was slow-growing, developmental-defective and severely impaired in erythromycin production. The results of genome-wide analysis of expression profiles using DNA micro-arrays demonstrated that these mutations deeply changed the transcriptional profile of S. erythraea with marked regional distribution. Keywords: mutants versus wild type comparison in a time course experiment

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Physiological responses of Saccharopolyspora erythraea towards perturbation of intracellular NADH/NAD+

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