Comparative Hi-C reveals that CTCF underlies evolution of chromosomal domain architecture.

ABSTRACT: Topological domains are key architectural building blocks of chromosomes in complex genomes. Their functional importance and evolutionary dynamics are however not well defined. Here we performed comparative Hi-C in liver cells from four mammalian species, and characterized the conservation and divergence of chromosomal contact insulation and the resulting domain architectures within distantly related genomes. We show that the modular organization of chromosomes is robustly conserved in syntenic regions. This overall conservation is compatible with conservation of the binding landscape of the insulator protein CTCF. Specifically, conserved CTCF sites are co-localized with cohesin, enriched at strong topological domain borders and bind to DNA motifs with orientations that define the direct

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Suzana Hadjur 

PROVIDER: E-GEOD-65126 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress